Application of ZHANG Zhongjing，s Jizihuang （Egg Yolk） Prescriptions in Type 2 Diabetes Mellitus based on the Theory of “Yin Restricts Fire” and “Many Different Diseases can be Treated in the Same Way” / 中医杂志

Type 2 diabetes mellitus （T2DM） can be categorized into “xiao ke （消渴）” in traditional Chinese medicine （TCM）. The theory of “yin restricts fire” originates from Inner Canon of Yellow Emperor （《黄帝内经》） which states that “yin essence restricts chief fire”， and the crucial pathogenesis and treatment of xiao ke coincide with this theory. ZHANG Zhongjing，s three prescriptions of Jizizhuang （egg yolk） are Baihe Jizizi Decoction （百合鸡子汤）， Huanglian Ejiao Decoction （黄连阿胶汤） and Painong Powder （排脓散）， which are scattered in different chapters of Treatise on Cold Damage and Miscellaneous Diseases （《伤寒杂病论》）. By analyzing and summarizing the mechanism and characteristics of the three prescriptions， it is found that the three prescriptions are in line with the characteristics of “yin restricts fire” and the pathogenesis of T2DM. These three prescriptions are composed of Jizizhuang and different medicinals. Baihe Jizizi Decoction is composed of Jizizhuang and Baihe （Bulbus Lilii）， and can be used to treat T2DM and mental diseases. Huanglian Ejiao Decoction is composed of Jizihuang， Ejiao （Colla Corii Asini）， Shaoyao （Radix Paeoniae Alba seu Rubra）， Huanglian （Rhizoma Coptidis） and Huangqin （Radix Scutellariae）， which could be used to treat T2DM and cardiorenal system diseases. Painong Powder is composed of Jizizhuang， Shaoyao， Jiegeng （Radix Platycodonis） and Zhishi （Fructus Aurantii Immaturus）， which can be used to treat T2DM and carbuncle. Therefore， based on the theory of “yin restricts fire” and “many different diseases can be treated in the same wa”， this paper propose that the three Jizihuang prescriptions could be used in T2DM， which could provide ideas for clinical treatment.

Huanglian Ejiao Decoction formula syndrome and its application for refractory insomnia / 中国中药杂志

Huanglian Ejiao Decoction,firstly recorded in Treatise on Febrile Diseases,could be used to treat heat-conversion syndrome of Shaoyin. It has been identified that,the indications of Huanglian Ejiao Decoction include:(1)in modern medicine,it can be used to treat insomnia,arrhythmia,oral ulcer,Xiali,blood syndrome and other diseases;(2)in terms of symptoms and signs,it is mainly used for restlessness,ritability,insomnia,extremely difficult to fall asleep,palpitation,atrial premature beat,ventricular premature beat and other arrhythmias,stomach distension and stuffiness,stomach pain,vomiting,abdominal pain,cramps,lower edge,constipation,blush,red lips,easy bleeding,red tongue,thin or no coating,dry tongue surface,or light red tongue,or dark red,thin white tongue coating,and rapid pulse. It has been also identified that,(1)although the original statement of Huanglian Ejiao Decoction is very simple,it can not only be used according to irritability and insomnia;(2)tongue image is not the key to syndrome differentiation of this prescription;(3)the essence of Shaoyin syndrome is not completely heart kidney Yang deficiency,which is closest to shock in modern medicine,including septic shock,hypovolemic shock and cardiogenic shock;(4)the essential difference between Shaoyin cold and heat conversion syndrome of Shaoyin lies in the different types of shock,in which cardiogenic shock is the main cold type,while septic shock is the main heat shock;(5)heat-conversion syndrome of Shaoyin is more common in the late stage of septic shock,and part of it can be seen in the stage of heart failure combined with sympathetic activation;(6)Dioscoreae Rhizoma and Rehmanniae Radix are often used to replace egg yolk;(7)Huanglian Ejiao Decoction takes effect quickly and the course of treatment is short.

Endoparasites of donkeys (Equus asinus) used in commercial skin exploitation in the Northeast of Brazil / Endoparasitos de jumentos (Equus asinus) utilizados em uma exploração comercial de peles no Nordeste brasileiro

The present study identified the main endoparasites present in donkeys (Equus asinus) used in skin exploitation located in Cando municipality, Bahia State, Northeast of Brazil. The samples were collected from September 2019. Feces were collected from the rectal ampulla of 34 animals, macroscopically visualized for parasitic forms, and microscopically evaluated to identify endoparasites forms using the McMaster method. Parasitological results were associated with sex, age, and bodyweight Stata Corp LLC 14. Endoparasites were found in 82.3% of the animals, with the egg count ranging from 50 to 1050 eggs per gram (EPG). The sole presence of superfamily Trichostronglylidae was observed in 67.6% of the donkeys, in 8.8% co-infected by Trichostronglylidae and Eimeria spp., while Trichostronglylidae and Strongyloides westeriwas detected in 2.9%, and simultaneous infection by Trichostronglylidae, Strongyloides westeri, and Oxyuris equi was observed in 2.9%. The occurrence of parasitic infections varies according to nutritional status, age, sex, and environmental exposure (p>0.05). A high occurrence of infection was observed in young animals and those with lower body weight.(AU)

O presente trabalho identificou os principais endoparasitas em jumentos utilizados em uma exploração de pele localizada no município de Canudos, Estado da Bahia, Nordeste do Brasil. As amostras foram colhidas no período de setembro de 2019. Fezes de 34 jumentos, colhidas diretamente da ampola retal, foram visualizadas macroscopicamente para formas parasitárias e avaliadas microscopicamente para identificar endoparasitos pela técnica de McMaster. Os resultados parasitológicos foram associados com sexo, idade e peso corporal pelo Stata Corp LLC 14. Endoparasitas foram encontrados em 82,3% dos animais, com contagem de ovos variando de 50 a 1050 ovos por grama (OPG). Presença de unicamente parasitos da superfamília Trichostronglylidae foi observada em 67,6% dos jumentos, 8,8% estavam coinfectados por Trichostronglylidae e Eimeria spp., 2,9% por Trichostronglylidae e Strongyloides westeri e 2,9% apresentaram presença simultânea de Trichostronglylidae, Strongyloides westeri e Oxyuris equi. A ocorrência de infecções parasitárias varia de acordo com a idade, sexo e exposição ambiental (p>0.05). Observou-se alta ocorrência de animais infectados nos animais jovens e naqueles com menor peso corporal.(AU)

The reality of the donkey's exploitation for the hide trade in Brazil: disease outbreaks and animal welfare compromised in rescued donkeys / A realidade da exploração de jumentos para exportação de pele no Brasil: surtos sanitários e bem-estar animal comprometido em asininos resgatados

About 800 donkeys that were confined in a restrictive area used in a manner comparable to a warehouse for receiving donkeys for slaughter were abandoned. After receiving reports of mistreatment, civilians acted to save the animals. A task force was organized that planned veterinary and zootechnical actions and activities for daily health management, feeding, and clinical care to attend to the abandoned donkeys. Positive cases were diagnosed for glanders, equine infectious anemia, equine herpesvirus, and equine babesiosis. The objective of this communication is to bring to the attention of the scientific community the interventions in the area of animal health and welfare, to address the episode of northeast donkeys that were victims of international trade. It is fundamental to change the approach related to the management of donkeys in Brazil, and appeal to the necessity to identify ethical and sustainable ways to incorporate donkeys in Brazil in the 21st century.(AU)

Cerca de 800 jumentos confinados em uma área utilizada como entreposto de recebimento e encaminhamento de jumentos para abate foram abandonados. Após denúncias de maus tratos, a sociedade civil atuou para salvar os animais. Foi constituída uma força tarefa que planejou ações e atividades veterinárias e zootécnicas atuando diariamente no manejo sanitário, na alimentação e nos cuidados clínicos na assistência aos jumentos abandonados. Foram diagnosticados casos positivos de mormo, anemia infecciosa equina, herpesvírus equino e babesiose equina. O objetivo desta comunicação foi relatar para a comunidade científica as intervenções nas áreas de saúde e bem-estar animal no episódio dos jumentos nordestinos vítimas do comércio internacional. É fundamental mudar a abordagem em relação ao manejo de jumentos no Brasil e apelar para a necessidade de identificar maneiras éticas e sustentáveis de incorporar os jumentos no Brasil do século 21.(AU)

Clinical efficacy evaluation and potential mechanism of Huanglian Ejiao Decoction in treatment of type 2 diabetes mellitus by Meta-analysis associated network pharmacology / 中草药

Objective: To evaluate the clinical efficacy of Huanglian Ejiao Decoction and explore its mechanism in the treatment of type 2 diabetes (T2DM) based on Meta-analysis and network pharmacology technology. Methods: Firstly, CNKI, VIP, Wanfang, PubMed, the Cochrane Library, and Web of Science were used to find randomized controlled trials of Huanglian Ejiao Decoction for T2DM until May 2020. The included literatures were evaluated according to the Cochrane risk bias assessment tool, and the Review Manager software was used for quantitative synthesis. The chemical components and its targets of Huanglian Ejiao Decoction were screened based on network pharmacology. Common targets were obtained after taking the intersection with T2DM targets. The bioinformatics technology was used to annotate key pathways, and the potential mechanism of Huanglian Ejiao Decoction was explored in the treatment of T2DM. Results: A total of 15 RCTs were selected which included 1 254 patients. Compared with the conventional treatment of western medicine, Huanglian Ejiao Decoction combined with western medicine had a more satisfactory clinical effect, stronger hypoglycemic and hypolipidemic effect. Through network pharmacology methods, 258 chemical components and 985 targets were obtained from online databases. There were 60 components, 121 targets, and 181 pathways closely related to the treatment of T2DM, mainly involving AGE-RAGE signaling pathway in diabetic complications, HIF-1 signaling pathway, MAPK signaling pathway, insulin resistance, etc. Conclusion According to the existing research, the conventional treatment of western medicine combined with Huanglian Ejiao Decoction can increase the clinical efficacy of T2DM and have better hypoglycemic and hypolipidemic action. T2DM can be cured by improving insulin resistance, reducing inflammation, mitigating oxidative stress and other treatments.

Herbalogical study of Colla Corii Asini(Ejiao) / 中国中药杂志

Colla Corii Asini(Ejiao)is an important Chinese medicine used in China for thousands of years, and is well known for its famous tonic properties. The herbalogical study was detailed carried out based on the naming, habitat, harvesting, processing, medicinal properties and clinical efficacy. The results showed that the name of Ejiao could be traced back to Shennong's Materia Medica, and various names of Lvpi Jiao, Penfu Jiao and Fuzhi Jiao were recorded in other ancient books. In the many intervening centuries, the main materials of Ejiao had been replaced from cow leather before Tang Dynasty to donkey skin in the middle to late Tang Dynasty. This phenomenon could be probably caused by complicated social factors of various periods and different efficacy of Ejiao made by all kinds of raw materials. Ejiao was merely processed with the simple methods before Tang Dynasty, which subsequently improved avariety of methods to enhance the supplementation action. Most importantly, Ejiao has a wide clinic application along with the development of processing theories and methods, which can be found in various Classics, especially in imperial medical case record in Qing Dynasty.

Mechanism of Huanglian Ejiao Tang on Prevention and Treatment of Myocardial Injury Induced by Anthracycline Chemotherapy Drugs / 中国实验方剂学杂志

Objective: To observe the preventive and therapeutic effect of Huanglian Ejiao Tang on myocardial injury induced by anthracycline chemotherapeutic drugs in all kinds of cancer patients undergoing chemotherapy. Method:We chosen all kinds of cancer patients with combined use of anthracycline chemotherapy drugs in our hospital, 21 days as one cycle. The cardiac toxicity reaction was observed after three continuous chemotherapy cycles. A total of 64 patients who met the dialectical criteria of "imbalance between heart-Yang and kidney-Yin" were randomly divided into treatment group (32 cases) and control group (32 cases). Patients in treatment group were treated with Chinese medicine Huanglian Ejiao Tang based on original chemotherapy regimen, adding and subtracting Chinese medical materials according to the symptoms. Patients in control group continued to maintain the original chemotherapy regimen, and both two groups of patients continued to receive 3 cycles of continuous chemotherapy. By comparing the cardiac function classification and cardiac function tolerance between the 3 cycles and 6 cycles of two groups of patients after chemotherapy; changes of echocardiography index, QTc interval, creatine kinase isoenzyme (CK-MB), Myoglobin (MYO), cardiac troponin I (cTNI)and nitrogenous terminal-pro-brain natriuretic peptide(NT-pro-BNP) concentration value were compared between two groups; and the concentrations of adrenaline (E), norepinephrine (NE) and angiotensin Ⅱ(AngⅡ) were observed and compared; meanwhile, the correlation analysis was carried out at the same time. Result:After 6 cycles of chemotherapy in Chinese medicine treatment group, degree of cardiac function classification and the 6 minute walking heart function tolerance were significantly better than those at the 3 cycles of chemotherapy (PPPPPConclusion:Huanglian Ejiao Tang can reduce the excitability of the symppthetic nervous system (SNS) and renin-angiotensin system(RAS) in human body and inhibit the release level of NE, E and AngⅡ by effect of "invigorating the kidney and clearing the heart". It has a certain preventive and treatment effect on the cardiac toxicity of patients with the cumulative use of anthracycline chemotherapy. To a certain extent, it can inhibit myocardial injury, improve cardiac function and reduce the incidence of cardiovascular events.

Research progress on identification and quality evaluation of glues medicines / 中国中药杂志

Glues medicines is a special kind of traditional Chinese medicine.As the market demand is large, the raw materials are in short supply and lacks proper quality evaluation technology, which causes inconsistent quality of products on the market. Its authentic identification and evaluation stay a problem to be solved. In this paper, the research progress of the methods and techniques of the evaluation of the identification and quality of glues medicines were reviewed. The researches of medicinal glue type identification and quality evaluation mainly concentrated in four aspects of medicinal materials of physical and chemical properties, trace elements, organic chemicals and biological genetic methods and techniques. The methods of physicochemical properties include thermal analysis, gel electrophoresis, isoelectric focusing electrophoresis, infrared spectroscopy, gel exclusion chromatography, and circular dichroism. The methods including atomic absorption spectrometry, X-ray fluorescence spectrometry, plasma emission spectrometry and visible spectrophotometry were used for the study of the trace elements of glues medicines. The organic chemical composition was studied by methods of composition of amino acids, content detection, odor detection, lipid soluble component, organic acid detection. Methods based on the characteristics of biogenetics include DNA, polypeptide and amino acid sequence difference analysis. Overall, because of relative components similarity of the glues medicines (such as amino acids, proteins and peptides), its authenticity and quality evaluation index is difficult to judge objectively, all sorts of identification evaluation methods have different characteristics, but also their limitations. It indicates that further study should focus on identification of evaluation index and various technology integrated application combining with the characteristics of the production process.

Clinical effect of Ejiao Huangqi oral liquid combined with norethisterone in treatment of dysfunctional uterine bleeding and its effect on improving anemia / 中国基层医药

Objective To observe the clinical effect of Ejiao Huangqi oral liquid combined with norethis-terone in treating dysfunctional uterine bleeding and its effect on improving anemia .Methods 100 patients with dysfunctional uterine bleeding were randomly divided into study group ( 50 cases ) and control group ( 50 cases ) according to the order of admission .The control group was given oral norethindrone treatment ,the study group was given Ejiao Huangqi oral liquid on the basis of the control group ,10mL per time,2 times a day.Both two groups were treated for 3 menstrual cycles.The fasting blood samples were taken to test the red blood cells (RBC) and hemoglobin(Hb). All patients were followed up for 3 months to evaluate clinical efficacy .Results The levels of RBC and Hb in the study group and control group were (3.05 ±0.58) ×1012/L and (3.67 ±0.65) ×1012/L,(86.75 ±6.30) g/L and (97.95 ±6.53) g/L,respectively,which were significantly higher than those before treatment (all P<0.05).The levels of RBC and Hb in the study group were significantly higher than those in the control group (P<0.05).The total effective rate of the study group was 92％(46/50),which was significantly higher than that of the control group [84％(42/50)],the difference was statistically significant (Z=-3.334,P<0.01).Conclusion Ejiao Huangqi oral liquid combined with norethisterone has exact effect in treating dysfunctional uterine bleeding , can quickly stop bleeding,improve the symptoms of anemia ,improve the clinical treatment effect ,which is worthy of clinical use .

Content Analysis and Comparative Study of Amino Acids in Ejiao from 18 Manufacturers / 中国药房

OBJECTIVE:To provide reference for quality evaluation of Ejiao. METHODS:The contents of 17 amino acids in 18 batches of Ejiao from 18 manufacturers were analyzed by automatic amino acid analyzer;the content data was processed by prin-cipal component analysis (PCA) and hierarchical clustering analysis (HCA),in order to understand the relationship among these amino acids and categorize the Ejiao products. RESULTS:Except for a batch of Ejiao,the other 17 batches contained 17 various amino acids,including 7 necessary amino acids(Thr,Val,Met,Ile,Leu,Phe and Lys)for human being,especially the contents of Gly and Pro were higher. The total content of amino acids was arranged from 66.1% to 82.0%,showing great difference. Four main components were extracted by PCA,the cumulative contribution of which was 89.578%,and accordingly it may be consid-ered that Pro,Ala,Glu,Asp,Leu,Ile and Thr were characteristic amino acids of Ejiao products. In hierarchical clustering analy-sis,3 tree figures of commercial Ejiao product were achieved and Euclidean distance was varied among 0-25. CONCLUSIONS:There is great difference in total control of amino acid in commercial Ejiao product;Ejiao products from various manufacturers dif-fer in their amino acid contents. Ejiao is rich in collagen,and therefore,using amino acid determination would be helpful to moni-tor its quality.

Qualitative Detection of Donkey-hide Gelatin in Fufang Ejiao Buxue Granule by UPLC-QQQ/MS / 中国药师

Objective:To establish an analytical method for the identification of donkey-hide gelatin in Fufang Ejiao Buxue gran-ule.Methods:The identification of donkey-hide gelatin in Fufang Ejiao Buxue granule was established by rapid resolution liquid chro -matography (UPLC) coupled with triple quadruple mass spectrometry (QQQ-MS).Results: The characteristic molecular peaks of donkey-hide gelatin were detected in ten batches of commercial samples .Conclusion:The present method is specific , precise and reli-able, and suitable for the identification of donkey-hide gelatin in Fufang Ejiao Buxue granule .The method provides scientific reference for the study of quality control method for gelatin ingredients in Chinese patent medicines .

Observation of the effect of ejiao huangqi koufuye and iron sucrose injection in the treatment of postpartum anemia / 中国基层医药

Objective To observe the effect of ejiao huangqi koufuye and iron sucrose injection in the treat-ment of postpartum anemia,to provide reference for clinical treatment.Methods 100 postpartum anemia women met the inclusion were randomly divided into 50 cases of observation group and 50 cases of control group.The control group was given iron sucrose injection 200mg for 3d,the observation group was given ejiao huangqi koufuye for 30d, twice daily and 10ml once.The blood was exsanguinated for detecting RBC,Hb,HCT,SF,TIBC.The improvement of symptoms and signs were observed and the effect was assessed.Results The RBC,Hb,HCT,SF of the observation group and control group after treatment were (2.14 ±0.74)×10 12 /L and (3.32 ±1.15 )×10 12 /L,(97.85 ± 22.44)g/L and (109.72 ±18.54)g/L,(36.42 ±8.39)% and (41.10 ±7.38)%,(22.26 ±1.76)mg/L and (32.2 ±2.1)mg/L,which were significantly higher than before treatment (t =3.065 and 5.614,3.411 and 3.961, 3.317 and 5.062,6.448 and 9.332,both P 0.05).Conclusion The ejiao huangqi kou-fuye and iron sucrose injection has exact clinical efficacy in the treatment of postpartum iron deficiency anemia,can effectively promote bone marrow erythropoiesis,anemia symptoms,it is worthy of clinical use.

Construction of NIRS-based total quality control system for compound Ejiao oral liquid and relative thinking / 中国中药杂志

In this paper, near infrared spectroscopy (NIRS)-based total quality control system of compound Ejiao oral liquid is introduced briefly, including the quality control of raw traditional Chinese medicine (TCM) materials, monitoring and control of the extract and the alkaline precipitation technics, and also the inspection of finished products in both open bottle and non-opening modes. By analyzing and summing up the significance and difficulties, several important problems in the practical applications of NIRS technology are proposed, which will provide references for the similar studies of other TCM products.

Research on analysis method for chemical variability of E'jiao based on 1H-NMR metabolomics / 中草药

Objective: The variability of the acid hydrolysis ingredients of E'jiao from different manufacturers was analyzed with the 1H-NMR-based metabolomics approach. Methods: E'jiao from five different manufacturers was hydrolyzed using acid and then analyzed using 1H-NMR. The component belongings were recognized and analyzed combined with relevant software for the multivariate statistical analysis, and then difference components were figured out. Results: Seventeen substances were recognized in the 1H-NMR profiling. After comparing E'jiao from manufacturers A, C, D, and E with that from manufacturer B respectively, the difference components including isoleucine, hydroxyproline, arginine acid, leucine, phenylalanine, valine, lysine, and levulinate were confirmed. Conclusion: 1H-NMR-based metabolomic study on E'jiao from different manufacturers could be used to analyze the difference components, and provide the new way and reasonable idea for quality control study of E'jiao.

Study on dry granulation technology for Ejiao granules / 中草药

Objective: To study the optimal technological conditions of Ejiao granules by dry granulation. Methods: The effects of rolling wheel press, speed, and powder feeding rate on grain yield were studied, and the dry granulation technology for Ejiao granules was optimized using L9(34) orthogonal test. Results: The optimal granulating conditions for the roll pressure was 60-70 kgf/cm2, powder feeding rate was 17-19 r/min, and rolling speed was 10-12 r/min. Conclusion: The study on the dry granulation technology for Ejiao granules provides the experimental basis for industrial application and spread of dry granulation technology in Chinese animal medicines.

Rapid determination of total flavonoids, total saponins, and soluble solid content in Fufang Ejiao Syrup by NIRS / 中草药

Objective: To develop an expeditious method for the simultaneous determination of main active compounds in Fufang Ejiao Syrup (FES, compound Ejiao oral liquid) by near infrared spectroscopy (NIRS) in transflective mode. Methods: The contents of total flavonoids and total saponins were determined by colorimetric assays, while the soluble solid content was determined using a moisture analyzer as reference value. Partial least squares regression (PLSR) was employed for the calibration of regression models between NIRS and the indexes. Results: The correlation coefficients (R2) of the constructed models for total flavonoids, total saponins, and soluble solid content were 0.9798, 0.9660, and 0.9978, respectively, and the root mean square errors of prediction (RMSEP) were 69.6, 17.6 μg/mL, and 0.394%, respectively. Conclusion: The proposed method is fast and accurate, and facilitating the rapid analysis of main active components in FES.

Research on the impact of compound E-Jiao slurry (CEJS) plus cyclophosphamide in the inhibition rate of lewis pulmonary carcinoma in mice / 国际中医中药杂志

Objective To investigate the effects of Compound E-Jiao Slurry (CEJS) and Cyclophosphamide (CTX) on the inhibition of Lewis pulmonary carcinoma xenografts. Methods Tumor xenografts models were prepared and randomly divided into six groups by weight, including the control group,CTX, CEJS with the dosages of high, middle and low, with a total of ten mice in each group. The control mice were given normal saline 0.4ml by intragastric administration once daily, with a total of twelve times; The mice of CTX group were given CTX 0.2 ml by intraperitoneal injection every other day, with a total of six times; The mice of CEJS group were given CEJS 0.2 ml and saline 0.2 ml by intragastric administration; The mice of combination therapy group 1 were given CEJS 0.4 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day; The mice of combination therapy group 2 were given CEJS 0.2 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day; The mice of combination therapy group 3 were given CEJS 0.1 ml by intragastric administration once daily, and CTX 0.2 ml by intraperitoneal injection every other day. The weight of tumor xenografts were measured in the experiment,the inhibition rate of tumor xenografts were calculated according to the data after the dissection. Results The combination groups and CTX alone have an inhibition rate of over 50%, and the CEJS group 25.88%. In every experimental group, the weight of tumor showed statistical significance compared with the control (P0.05). Conclusion CEJS can't obviously enhance the sensitivity of tumor xenografts to CTX, but there is a trend for tumor inhibition.

Effects of compound E-Jiao Slurry(CEJS)on the expression of VEGF in mice with lewis lung carcinoma / 国际中医中药杂志

Objective To observe the effects of CEJS combined with chemotherapy on the expression of VEGF in lewis lung carcinoma.Methods Tumor xenografts models were set up and randomly divided into five groups including control group,CTX CEJS,high-dose CEJS joint CTX(combination group 1),and middle-dose CEJS joint CTX(combination group 2).Paraffin imbedding and HE staining were performed,and the tissue Shape was observed by microscope after section.The expressions of VEGF in five groups were determined by immuno-histochemistry.Results The expression level of VEGF could be significantly down-regulated in CTX or combination group 1 and 2,compared with the control group(P＜0.05):CEJS group had no significant difference to the control group(P＞0.05);The average optical density in the combination group 1 and 2 showed no significantly difference to the CTX group(P＞0.05).Conclusion The inhibition of CEJS could not down regulate of the expression of VEGF,but CEJS could keep the affection of chemotherapy drugs on VEGF.

Determination of AstragalosideⅣin Ejiao Buxue Koufuye by HPLC-ELSD / 中国中医药信息杂志

Objective To establish a method for the determination of AstragalosideⅣin Ejiao Buxue Koufuye.Methods HPLC-ELSD was used to determine directly AstragalosideⅣin Ejiao Buxue Koufuye on Betasil C_(18) column,using CH_3OH-H_2O(74:26)as a mobile phase.The flow rate was 1.0mL/min.The temperature of drift tube for ELSD was 90℃and the flow rate of N_2 was 2.0L/min.Results The linear range of AstragalosideⅣwas 0.43～2.15?g,Y=1.53637X+0.92158,r=0.9999(n=6),the average recovery of the added sample was 98.5% and RSD=0.98%.Conclusion The method is accurate and reliable with a good reproducibility.It can be used for the quality control of Ejiao Buxue Koufuye.

Determination of copper,zinc and maganese in Ejiao by derivative flame atomic absorption spectrometry / 中国药学杂志

OBJECTIVE:To determine the contents of Cu,Zn and Mn in Ejiao,a kind of Chinese traditional medicine.METHOD:The contents of Cu,Zn and Mn were determined by derivative flame atomic absorption spectrometry (DFAAS) after digested by HNO3-HClO4.RESULTS:The contents of Cu,Zn and Mn were 10.48,12.38 and 18.09μg.g-1 respectively.CONCLUSIONS:The DFAAS possesses higher sensitivities,lower detection limits and better precision.